Genetic Transformation Systems in Fungi, Volume 2
Genetic Transformation Systems in Fungi, Volume 2 [electronic resource] /
edited by Marco A. van den Berg, Karunakaran Maruthachalam.
- XIV, 270 p. 38 illus., 26 illus. in color. online resource.
- Fungal Biology, 2198-7777 .
- Fungal Biology, .
Anastomosis and Heterokaryon Formation -- Induction of the Sexual Cycle in Filamentous Ascomycetes -- What Have We Learned by Doing Transformations in Neurospora tetrasperma? -- Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process -- Calculating RIP Mutation in Fungal Genomes Using RIPCAL -- Fungal Transposable Elements -- In vivo Targeted Mutagenesis in Yeast using TaGTEAM -- RNA Silencing in Filamentous Fungi: from Basic to Applications -- RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum -- Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigates -- Expanding the Repertoire of Selectable Markers for Aspergillus Transformation -- Arginase (agaA) as a Fungal Transformation Marker -- Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors -- A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma -- Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi -- Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals -- Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi -- Yeast Cell Electroporation in Droplet-Based Microfluidic Chip -- Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis -- Genetic and Genomic Manipulation in Aspergillus niger -- Genetic Manipulation of Meyerozyma guilliermondii.
Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. While Volume 1 provided its readers with an in-depth understanding of how the transformation of DNA is used to understand the genetic basis behind these fungal traits, Volume 2 of this text describes the transformation associated methods, tools, and many relevant elements available to the modern fungal researcher. With ample illustrative examples and protocols, Genetic Transformation Systems in Fungi, Volume 2 is meant not only as a reference guide for the experienced researcher, but also as an a learning tool for the emerging scientist.
9783319105031
10.1007/978-3-319-10503-1 doi
Life sciences.
Systems biology.
Developmental biology.
Plant genetics.
Plant pathology.
Plant breeding.
Life Sciences.
Developmental Biology.
Plant Genetics & Genomics.
Systems Biology.
Plant Breeding/Biotechnology.
Plant Pathology.
QH491
571.8
Anastomosis and Heterokaryon Formation -- Induction of the Sexual Cycle in Filamentous Ascomycetes -- What Have We Learned by Doing Transformations in Neurospora tetrasperma? -- Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process -- Calculating RIP Mutation in Fungal Genomes Using RIPCAL -- Fungal Transposable Elements -- In vivo Targeted Mutagenesis in Yeast using TaGTEAM -- RNA Silencing in Filamentous Fungi: from Basic to Applications -- RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum -- Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigates -- Expanding the Repertoire of Selectable Markers for Aspergillus Transformation -- Arginase (agaA) as a Fungal Transformation Marker -- Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors -- A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma -- Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi -- Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals -- Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi -- Yeast Cell Electroporation in Droplet-Based Microfluidic Chip -- Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis -- Genetic and Genomic Manipulation in Aspergillus niger -- Genetic Manipulation of Meyerozyma guilliermondii.
Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. While Volume 1 provided its readers with an in-depth understanding of how the transformation of DNA is used to understand the genetic basis behind these fungal traits, Volume 2 of this text describes the transformation associated methods, tools, and many relevant elements available to the modern fungal researcher. With ample illustrative examples and protocols, Genetic Transformation Systems in Fungi, Volume 2 is meant not only as a reference guide for the experienced researcher, but also as an a learning tool for the emerging scientist.
9783319105031
10.1007/978-3-319-10503-1 doi
Life sciences.
Systems biology.
Developmental biology.
Plant genetics.
Plant pathology.
Plant breeding.
Life Sciences.
Developmental Biology.
Plant Genetics & Genomics.
Systems Biology.
Plant Breeding/Biotechnology.
Plant Pathology.
QH491
571.8