Editorial and Introduction to Volume 2 -- Part I Novel nanocarrier design and processing -- Proprietary nanofiber technologies and scale-up -- Magnetically responsive (nano)biocomposites.-Zwitterionic nanocarriers for gene delivery -- Stimuli-responsive polymeric nanocarriers as promising drug and gene delivery systems --  Photo-responsive polymeric nanocarriers for on-demand drug delivery -- Part II Nanocarrier characterization and function -- Considerations on uptake and intracellular trafficking of nanocarriers -- Mucus as physiological barrier to intracellular delivery -- Investigation of nanoparticles in biological objects by electron microscopy techniques -- Gold nanoparticles for high resolution imaging in modern immunocytochemistry -- Design of functional polymers for intracellular drug nucleic acids delivery -- Membrane-domain-selective drug targeting based on lipid modification -- Multifuntional protein-based nanoparticles for theragnosis -- Cytocompatible phospholipid polymer for noninvasive nanodevice -- Intracellular protein delivery using self-assembled amphiphilic polysaccharide nanogels -- Part III Simulation for delivery and function -- Molecular dynamics simulations of polyplexes and lipoplexes -- Computational studies of highly pegylated sterically stabilized micelles self-assembly and drug solubilization -- Toward intracellular delivery and drug discovery,Stochastic logic networks as efficient computational models for gene regulatory networks -- Part IV Nanocarriers for drug discovery and treatment -- Nanodiamonds as intracellular probes for imaging in biology and medicine -- Intracellular delivery of RNA using RNA-binding proteins or peptides -- Hyaluronic acid based nanofibers for wound dressing and drug delivery carriers -- Potential of siRNA therapy in chronic myeloid leukemia.

This volume is a continuation of Volume 1 following the previously published Editorial. More emphasis is given to novel nanocarrier designs, their characterization and function, and applications for drug discovery and treatment. A number of chapters will deal with nanofibers as a new major application within the biomedical field with a very high success rate particularly in wound healing and diabetic foot and spine injuries. A major new subdivision will deal with mathematical methods for the assembly of nanocarriers  both for simulation and function.