000 | 03118nam a22004935i 4500 | ||
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001 | 978-3-0348-0856-9 | ||
003 | DE-He213 | ||
005 | 20160405110542.0 | ||
007 | cr nn 008mamaa | ||
008 | 140805s2014 sz | s |||| 0|eng d | ||
020 |
_a9783034808569 _9978-3-0348-0856-9 |
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024 | 7 |
_a10.1007/978-3-0348-0856-9 _2doi |
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050 | 4 | _aQD431-431.7 | |
072 | 7 |
_aPSBC _2bicssc |
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072 | 7 |
_aSCI007000 _2bisacsh |
|
082 | 0 | 4 |
_a572.6 _223 |
245 | 1 | 0 |
_aFluorescent Methods for Molecular Motors _h[electronic resource] / _cedited by Christopher P. Toseland, Natalia Fili. |
264 | 1 |
_aBasel : _bSpringer Basel : _bImprint: Springer, _c2014. |
|
300 |
_aX, 298 p. 88 illus., 49 illus. in color. _bonline resource. |
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336 |
_atext _btxt _2rdacontent |
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337 |
_acomputer _bc _2rdamedia |
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338 |
_aonline resource _bcr _2rdacarrier |
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347 |
_atext file _bPDF _2rda |
||
490 | 1 |
_aExperientia Supplementum, _x1023-294X ; _v105 |
|
505 | 0 | _aFluorescence and Labelling: How to choose and what to do -- Fluorescent biosensors: design and application to motor proteins -- Rapid Reaction Kinetic Techniques -- Fluorescence to study the ATPase mechanism of Motor Proteins -- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies -- Fluorescent methods to study transcription initiation and transition into elongation -- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo -- Fluorescence methods in the investigation of the DEAD-box helicase mechanism -- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins -- Fluorescence Tracking of Motor proteins in vitro -- Measuring Transport of Motor Cargos -- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET -- Using fluorescence to study actomyosin in yeasts. | |
520 | _aThis book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments. | ||
650 | 0 | _aLife sciences. | |
650 | 0 | _aProteins. | |
650 | 0 | _aCell biology. | |
650 | 0 | _aBiophysics. | |
650 | 1 | 4 | _aLife Sciences. |
650 | 2 | 4 | _aProtein Science. |
650 | 2 | 4 | _aCell Biology. |
650 | 2 | 4 | _aSingle Molecule Studies, Molecular Motors. |
700 | 1 |
_aToseland, Christopher P. _eeditor. |
|
700 | 1 |
_aFili, Natalia. _eeditor. |
|
710 | 2 | _aSpringerLink (Online service) | |
773 | 0 | _tSpringer eBooks | |
776 | 0 | 8 |
_iPrinted edition: _z9783034808552 |
830 | 0 |
_aExperientia Supplementum, _x1023-294X ; _v105 |
|
856 | 4 | 0 | _uhttp://dx.doi.org/10.1007/978-3-0348-0856-9 |
912 | _aZDB-2-SBL | ||
999 |
_c2844 _d2844 |