000 03118nam a22004935i 4500
001 978-3-0348-0856-9
003 DE-He213
005 20160405110542.0
007 cr nn 008mamaa
008 140805s2014 sz | s |||| 0|eng d
020 _a9783034808569
_9978-3-0348-0856-9
024 7 _a10.1007/978-3-0348-0856-9
_2doi
050 4 _aQD431-431.7
072 7 _aPSBC
_2bicssc
072 7 _aSCI007000
_2bisacsh
082 0 4 _a572.6
_223
245 1 0 _aFluorescent Methods for Molecular Motors
_h[electronic resource] /
_cedited by Christopher P. Toseland, Natalia Fili.
264 1 _aBasel :
_bSpringer Basel :
_bImprint: Springer,
_c2014.
300 _aX, 298 p. 88 illus., 49 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aExperientia Supplementum,
_x1023-294X ;
_v105
505 0 _aFluorescence and Labelling: How to choose and what to do -- Fluorescent biosensors: design and application to motor proteins -- Rapid Reaction Kinetic Techniques -- Fluorescence to study the ATPase mechanism of Motor Proteins -- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies -- Fluorescent methods to study transcription initiation and transition into elongation -- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo -- Fluorescence methods in the investigation of the DEAD-box helicase mechanism -- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins -- Fluorescence Tracking of Motor proteins in vitro -- Measuring Transport of Motor Cargos -- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET -- Using fluorescence to study actomyosin in yeasts.
520 _aThis book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
650 0 _aLife sciences.
650 0 _aProteins.
650 0 _aCell biology.
650 0 _aBiophysics.
650 1 4 _aLife Sciences.
650 2 4 _aProtein Science.
650 2 4 _aCell Biology.
650 2 4 _aSingle Molecule Studies, Molecular Motors.
700 1 _aToseland, Christopher P.
_eeditor.
700 1 _aFili, Natalia.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783034808552
830 0 _aExperientia Supplementum,
_x1023-294X ;
_v105
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-0348-0856-9
912 _aZDB-2-SBL
999 _c2844
_d2844